BAX 抗体 (CF®740)
Quick Overview for BAX 抗体 (CF®740) (ABIN7650003)
抗原
See all BAX 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Bax(BAX/962), CF740 conjugate
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产品特性
- This antibody recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human BAX protein.
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亚型
- IgG1
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anti-BCL2-Associated X Protein (BAX) (AA 84-175) antibody
BAX 适用: 人, 小鼠, 大鼠, Pig, Cow, 兔, 小鸡, 斑马鱼 WB, ELISA, FACS, IP, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 1-100) antibodyBAX 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (N-Term) antibodyBAX 适用: 人, 小鼠, 大鼠, 猴, Pig, Cow, 兔, 犬, 小鸡, 绵羊, 马, 豚鼠, 山羊, Hamster, Avian, Cat, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 1-158) antibodyBAX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 1-192) antibodyBAX 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 1F5-1B7 unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 13-160) antibodyBAX 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 4H9B11 unconjugated
anti-BCL2-Associated X Protein (BAX) antibodyBAX 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2G6B2 unconjugated
anti-BCL2-Associated X Protein (BAX) antibodyBAX 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 2D8H2 unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 41-76) antibodyBAX 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB0952 unconjugated
anti-BCL2-Associated X Protein (BAX) (N-Term) antibodyBAX 适用: 人, Pig, Cow, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Does not react with mouse or rat, others not known. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- BAX (BCL2-Associated X Protein (BAX))
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别名
- Bax
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背景
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Synonyms: BAX, Apoptosis regulator BAX, BCL2 associated X protein, Bcl-2-like protein 4
Gene Symbol: BAX
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分子量
- 21 kDa
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基因ID
- 581
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UniProt
- Q07812
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途径
- p53 Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
抗原
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