SGK1 抗体 (N-Term)
Quick Overview for SGK1 抗体 (N-Term) (ABIN7603118)
抗原
See all SGK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Anti-SGK1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SGK1 Antibody Picoband® (ABIN7603118). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human SGK1, which shares 95.7% amino acid (aa) sequence identity with rat SGK1.
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亚型
- IgG
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anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) antibody
SGK1 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 4D7-G3 unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer422) antibodySGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 172-431) antibodySGK1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-90) antibodySGK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer78) antibodySGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 145-386) antibodySGK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 247-406) antibodySGK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-90) antibodySGK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3E3 unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pThr256) antibodySGK1 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer422) antibodySGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Arteaga, M. F., Coric, T., Straub, C., Canessa, C. M. A brain-specific SGK1 splice isoform regulates expression of ASIC1 in neurons. Proc. Nat. Acad. Sci. 105: 4459-4464, 2008. 2. Arteaga, M. F., Wang, L., Ravid, T., Hochstrasser, M., Canessa, C. M. An amphipathic helix targets serum and glucocorticoid-induced kinase 1 to the endoplasmic reticulum-associated ubiquitin-conjugation machinery. Proc. Nat. Acad. Sci. 103: 11178-11183, 2006. 3. Gamper, N., Fillon, S., Feng, Y., Friedrich, B., Lang, P. A., Henke, G., Huber, S. M., Kobayashi, T., Cohen, P., Lang, F. K+ channel activation by all three isoforms of serum- and glucocorticoid-dependent protein kinase SGK. Pflugers Arch. 445: 60-66, 2002. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
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别名
- SGK1
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背景
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Synonyms: Serine/threonine-protein kinase Sgk1, Serum/glucocorticoid-regulated kinase 1, SGK1, SGK
Tissue Specificity: Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
Background: Serine/threonine-protein kinase Sgk1 also known as serum and glucocorticoid-regulated kinase 1 is an enzyme that in humans is encoded by the SGK1 gene. It is mapped to 6q23.2. This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
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分子量
- 55 kDa
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基因ID
- 6446
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UniProt
- O00141
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途径
- MAPK Pathway, Notch Signaling, Steroid Hormone Mediated Signaling Pathway
抗原
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