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SGK1 抗体 (pSer422)

SGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of SGK phosphorylation expression in Insulin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining human skin tissue sections by IHC-P. (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining rat uterine tissue sections by IHC-P. (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining rat intestinal tissue sections by IHC-P. (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining mouse gastric tissue sections by IHC-P. (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining mouse testis tissue sections by IHC-P. (SGK1 抗体 (pSer422))

ABIN6267218 staining HeLa by IF/ICC. (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining human appendiceal tissue sections by IHC-P. (SGK1 抗体 (pSer422))

Western blot analysis of Phospho-SGK (Ser422) expression in various lysates (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining mouse lung tissue sections by IHC-P. (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining human TB tissue sections by IHC-P. (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining mouse spleen tissue sections by IHC-P. (SGK1 抗体 (pSer422))

ABIN6267218 at 1/100 staining rat ovarian tissue sections by IHC-P. (SGK1 抗体 (pSer422))

13 images

产品编号 ABIN6256329

发货至: 中国

Datasheet as PDF

抗原 See all SGK1 抗体

SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
抗原表位
20

19

16

14

9

6

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer422
适用
138

93

75

20

17

8

7

5

5

4

4

2

2

1

1
人, 小鼠, 大鼠
宿主
123

11

4

1
兔
Compatible Secondaries
克隆类型
127

12
多克隆
标记
66

9

8

7

5

4

4

4

4

4

4

4

4

4

3

3

2
This SGK1 antibody is un-conjugated
应用范围
131

56

50

44

40

29

27

21

9

7

3

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Phospho-SGK1 (Ser422) Antibody detects endogenous levels of SGK1 only when phosphorylated at Serine 422.

预测反应

Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human SGK1 around the phosphorylation site of Ser422.

亚型

IgG

Top Product

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anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) antibody
SGK1 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 4D7-G3 unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 172-431) antibody
SGK1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-90) antibody
SGK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3G8 unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer78) antibody
SGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 145-386) antibody
SGK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 247-406) antibody
SGK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-90) antibody
SGK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3E3 unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer422) antibody
SGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pThr256) antibody
SGK1 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (Tyr1062) antibody
SGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

别名

SGK1 (SGK1 产品)

别名

SGK antibody, Sgk antibody, sgk-B antibody, sgk2 antibody, cb1083 antibody, sgk antibody, wu:fc20a09 antibody, sgk-A antibody, sgk1 antibody, sgk1-a antibody, sgk1-b antibody, serum/glucocorticoid regulated kinase 1 antibody, Serine/threonine-protein kinase sgk-1 antibody, serum/glucocorticoid regulated kinase 1 S homeolog antibody, serum/glucocorticoid regulated kinase 1 L homeolog antibody, SGK1 antibody, Sgk1 antibody, sgk-1 antibody, sgk1.S antibody, sgk1 antibody, sgk1.L antibody

背景

Description: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na+ retention, renal K+ elimination, salt appetite, gastric acid secretion, intestinal Na+/H+ exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na+ channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K+ channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca2+ channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na+/dicarboxylate cotransporter: SLC13A2/NADC1, Na+-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na+/K+ ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1. Regulates KCNJ1/ROMK1 directly via its phosphorylation or indirectly via increased interaction with SLC9A3R2/NHERF2. Phosphorylates MDM2 and activates MDM2-dependent ubiquitination of p53/TP53. Phosphorylates MAPT/TAU and mediates microtubule depolymerization and neurite formation in hippocampal neurons. Phosphorylates SLC2A4/GLUT4 and up-regulates its activity. Phosphorylates APBB1/FE65 and promotes its localization to the nucleus. Phosphorylates MAPK1/ERK2 and activates it by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Phosphorylates FBXW7 and plays an inhibitory role in the NOTCH1 signaling. Phosphorylates FOXO1 resulting in its relocalization from the nucleus to the cytoplasm. Phosphorylates FOXO3, promoting its exit from the nucleus and interference with FOXO3-dependent transcription. Phosphorylates BRAF and MAP3K3/MEKK3 and inhibits their activity. Phosphorylates SLC9A3/NHE3 in response to dexamethasone, resulting in its activation and increased localization at the cell membrane. Phosphorylates CREB1. Necessary for vascular remodeling during angiogenesis. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Isoform 2 exhibited a greater effect on cell plasma membrane expression of SCNN1A/ENAC and Na+ transport than isoform 1.
Gene: SGK1

分子量

54kDa

基因ID

6446

UniProt

O00141

途径

MAPK Pathway, Notch Signaling, Steroid Hormone Mediated Signaling Pathway

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