Collagen IV 抗体
Quick Overview for Collagen IV 抗体 (ABIN7565969)
抗原
See all Collagen IV (COL4) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Collagen Type IV Antibody
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交叉反应 （详细）
- Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
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产品特性
- Synonyms: rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1
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纯化方法
- This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Collagen Type IV from human and bovine placenta
Immunogen Type: Native Protein
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anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人, Cow WB, ELISA, IHC, IP, DB, FM 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1571-1669) antibodyCOL4 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1401-1500) antibodyCOL4 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA, IHC, IHC (fro), IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-704R unconjugated Recombinant Antibody
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated
anti-Collagen, Type IV (COL4) antibody (FITC)COL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 FITC
anti-Collagen, Type IV (COL4) antibody (Biotin)COL4 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
- Application Note: Anti-Collagen Type IV has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis. Immunohistochemistry Dilution: 1:50 - 1:200 Western Blot Dilution: 1:1,000 - 1:10,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:50,000 IF Microscopy Dilution: User Optimized Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.18 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Collagen IV (COL4) (Collagen, Type IV (COL4))
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别名
- Collagen Type IV
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背景
- Background: Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
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基因ID
- 1282
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NCBI登录号
- NP_001290039
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UniProt
- P02462
抗原
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