MEK1 抗体 (C-Term) (Biotin)
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Quick Overview for MEK1 抗体 (C-Term) (Biotin) (ABIN7565793)
抗原
See all MEK1 (MAP2K1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- MEK1 C-Term Antibody Biotin
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交叉反应 （详细）
- This biotin conjugated protein A purified mouse monoclonal antibody reacts specifically with human MEK1.
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产品特性
- Synonyms: mouse anti-MEK1 antibody biotin conjugation, biotin conjugated mouse anti-MEK1 antibody, MAP2K, MEK, MEK 1, MKK1, PRKMK1, MEK-1 Antibody
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纯化方法
- Anti-MEK1 is purified from tissue culture supernatant by protein A purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-MEK1 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
Immunogen Type: Conjugated Peptide
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亚型
- IgG1 kappa
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Labeling Ratio
- 10-20
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anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody
MAP2K1 适用: 人 ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-100) antibodyMAP2K1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 2H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibodyKD Validated MAP2K1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB285 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyKD Validated MAP2K1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB2475 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer221) antibodyMAP2K1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (N-Term) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr292) antibodyKD Validated MAP2K1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB305 unconjugated Recombinant Antibody
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应用备注
- Application Note: Anti-MEK 1 Biotin Conjugated (MOUSE) Antibody has been tested by Western Blotting and ELISA. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:40,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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别名
- MAP2K1
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背景
- Background: MEK1 antibodies detect the MEK1 isoform. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain. The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
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UniProt
- Q02750
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
抗原
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