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Insulin 抗体 (AA 46-59)

Name: Insulin 抗体 (AA 46-59)
SKU: ABIN724265
Availability: OutOfStock

INS 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Insulin Polyclonal Antibody, Unconjugated (ABIN724265) 1:200 followed by conjugation to the secondary antibody and DAB staining (Insulin 抗体 (AA 46-59))

IHC

Changes in adrenal glands and pancreas in acNTS injured mice. (Insulin 抗体 (AA 46-59))

IHC (p)

Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-Insulin Polyclonal Antibody, Unconjugated (ABIN724265) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C. (Insulin 抗体 (AA 46-59))

Mouse Pancreas (C57BL/6 strain) fixed in 4% PFA for 2 hours at RT, then transferred to 30% sucrose overnight at 4°C before embedding in OCT mounting medium. (Insulin 抗体 (AA 46-59))

IF (p)

4 images

(1 reference)

产品编号 ABIN724265

发货至: 中国

Datasheet as PDF

抗原 See all Insulin (INS) 抗体

Insulin (INS)
抗原表位
14

9

8

4

4

3

2

2

2

1

1

1

1

1

1

1

1
AA 46-59
适用
183

107

85

75

72

41

2

2

2

1

1

1

1

1

1

1

1

1
小鼠, 大鼠
宿主
121

85

11

1

1

1
兔
Compatible Secondaries
克隆类型
131

90

1
多克隆
标记
108

13

10

5

5

5

5

5

5

4

4

4

4

4

4

4

4

4

4

4

2

2

2

2

2

2

2

1

1

1
This Insulin antibody is un-conjugated
应用范围
67

64

63

60

52

47

47

42

35

17

17

17

14

9

6

6

3

3

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

小鼠, 大鼠

预测反应

Human,Cow,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Insulin

亚型

IgG
anti-Insulin (INS) antibody
INS 适用: 人 IHC, WB, IF 宿主: 兔 Monoclonal unconjugated

anti-Insulin (INS) (AA 90-110) antibody
INS 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin (INS) (AA 25-110) antibody
INS 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin (INS) antibody
INS 适用: 人, Pig, 小鼠, Cow, 大鼠, 兔, 豚鼠, 犬, 猴, Cat, 小鸡, 驴, 山羊, Hamster, 马, 绵羊, Avian WB 宿主: 山羊 Polyclonal unconjugated

anti-Insulin (INS) (AA 25-110) antibody
INS 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin (INS) (AA 25-110) antibody
INS 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal H10 unconjugated

anti-Insulin (INS) (AA 25-54) antibody
INS 适用: Cow IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin (INS) antibody
INS 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 1F6 unconjugated Recombinant Antibody

anti-Insulin (INS) antibody
INS 适用: 人, Pig, 小鼠, Cow, 兔 IHC, StM 宿主: 兔 Monoclonal IRDN-1980R unconjugated Recombinant Antibody

anti-Insulin (INS) antibody
INS 适用: 人, Pig, 小鼠, Cow IHC, IF, IHC (p), FACS, ISt, StM 宿主: 小鼠 Monoclonal 2D11-H5-same as INS05 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Yang, Zhao, Zhao, Yuan, Chen, Varghese, Moorhead, Chen, Ruan: "Paradoxical effect of rapamycin on inflammatory stress-induced insulin resistance in vitro and in vivo." in: Scientific reports, Vol. 5, pp. 14959, (2016) (PubMed).
抗原

Insulin (INS)

别名

Insulin (INS 产品)

背景

Synonyms: ILPR, IRDN, IDDM1, IDDM2, MODY1, Insulin, INS
Background: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

基因ID

3630

UniProt

P01308

途径

NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation