SQSTM1 抗体 (Internal Region)
(1 reference)
Quick Overview for SQSTM1 抗体 (Internal Region) (ABIN7540593)
抗原
See all SQSTM1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-hb8
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Supplier
- Rockland
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原理
- SQSTM1/p62 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human SQSTM1/p62.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-SQSTM1/p62 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SQSTM1 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Sequestosome 1 (SQSTM1) antibody
KO Validated SQSTM1 适用: 人 WB, IF, IP, FACS, ICC, IHC (p), PLA 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 51-150) antibodySQSTM1 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibodySQSTM1 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibodySQSTM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 232-356) antibodySQSTM1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H7E2 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 317-346) antibodySQSTM1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4614 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 136-400) antibodySQSTM1 适用: 人 WB, IHC, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (C-Term) antibodySQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) antibodySQSTM1 适用: 人, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Sequestosome 1 (SQSTM1) antibodyKO Validated SQSTM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000-1:50,000
Immunohistochemistry_Dilution: 1:100
IF_Microscopy_Dilution: 5 μg/mL
Western_Blot_Dilution: 1:1000
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说明
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Anti-SQSTM1/p62 Antibody has been tested in ELISA, WB, IF, and IHC. Expect a band at ~38, 47kDa in western blot using appropriate tissues and lysates. Positive control used: U87-MG, HeLa, and Mouse liver lysates in western blot, Human skeletal muscle in Immunohistochemistry, and HeLa in immunofluorescence.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Exploring the Interplay of RUNX2 and CXCR4 in Melanoma Progression." in: Cells, Vol. 13, Issue 5, (2024) (PubMed).
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: "Exploring the Interplay of RUNX2 and CXCR4 in Melanoma Progression." in: Cells, Vol. 13, Issue 5, (2024) (PubMed).
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- SQSTM1 (Sequestosome 1 (SQSTM1))
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别名
- SQSTM1
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背景
- Rabbit Anti-Sequestosome 1 Antibody, Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa, EBI3-Associated Protein Of 60 KDa, Ubiquitin-Binding Protein P62, Oxidative Stress Induced Like, EBIAP, OSIL, P60, Phosphotyrosine Independent Ligand For The Lck SH2 Domain P62, EBI3-Associated Protein P60, Paget Disease Of Bone, Sequestosome-1, FTDALS3, NADGP, A170, DMRV, ZIP3, P62B, PDB3, ORCA, P62,SQSTM1(Sequestosome 1)/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It functions as a bridge between polyubiquitinated cargo and autophagosomes and interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Along with WDFY3, SQSTM1 is involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures) and is required to recruit ubiquitinated proteins to PML bodies in the nucleus. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and may play a role in titin/TTN downstream signaling in muscle cells. It is an adapter that mediates the interaction between TRAF6 and CYLD. SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. It is involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Mutations in this gene result in frontotemporal dementia and/or amyotrophic lateral sclerosis, and familial Paget disease of bone. Anti-SQSTM1 Antibody is useful for researchers interested in Cancer Research, Neuroscience, and Cytokines & Growth Factor Antibodies.
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基因ID
- 8878
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NCBI登录号
- NP_001135770
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UniProt
- Q13501
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途径
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy
抗原
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