SQSTM1 抗体 (N-Term)
(5 references)-
- 抗原 See all SQSTM1 抗体
- SQSTM1 (Sequestosome 1 (SQSTM1))
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抗原表位
- AA 69-96, N-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SQSTM1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Rabbit IgG polyclonal antibody for Sequestosome-1(SQSTM1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
- 序列
- DEDGDLVAFS SDEELTMAMS YVKDDIFR
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Sequestosome-1(SQSTM1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: sequestosome 1
Protein Name: Sequestosome-1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62 (69-96aa DEDGDLVAFSSDEELTMAMSYVKDDIFR), identical to the related mouse and rat sequences.
- 亚型
- IgG
- Top Product
- Discover our top product SQSTM1 Primary Antibody
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anti-Sequestosome 1 (SQSTM1) antibody
KO Validated SQSTM1 适用: 人 WB, IF, IP, IHC (p), ICC, FACS, PLA 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 51-150) antibodySQSTM1 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibodySQSTM1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibodySQSTM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 232-356) antibodySQSTM1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H7E2 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 317-346) antibodySQSTM1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4614 unconjugated
anti-Sequestosome 1 (SQSTM1) (AA 136-400) antibodySQSTM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) (C-Term) antibodySQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sequestosome 1 (SQSTM1) antibodySQSTM1 适用: 人 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 4E2 unconjugated
anti-Sequestosome 1 (SQSTM1) antibodySQSTM1 适用: 人, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 5A2 unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for SQSTM1/p62 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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: "Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice." in: Scientific reports, Vol. 7, pp. 43684, (2018) (PubMed).
: "Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication." in: Archives of virology, Vol. 163, Issue 1, pp. 135-144, (2018) (PubMed).
: "Dual attenuation of proteasomal and autophagic BMAL1 degradation in Clock Δ19/+ mice contributes to improved glucose homeostasis." in: Scientific reports, Vol. 5, pp. 12801, (2017) (PubMed).
: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, (2017) (PubMed).
: "Disturbed Flow Induces Autophagy, but Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis." in: Antioxidants & redox signaling, Vol. 23, Issue 15, pp. 1207-19, (2016) (PubMed).
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: "Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice." in: Scientific reports, Vol. 7, pp. 43684, (2018) (PubMed).
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- 抗原
- SQSTM1 (Sequestosome 1 (SQSTM1))
- 别名
- SQSTM1 (SQSTM1 产品)
- 背景
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SQSTM1(Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.
Synonyms: A170 antibody|EBI 3 associated protein of 60 kDa antibody|EBI 3 associated protein p60 antibody|EBI3 associated protein of 60 kDa antibody|EBI3 associated protein p60 antibody| EBI3-associated protein of 60 kDa antibody|EBIAP antibody|MGC127197 antibody|ORCA antibody| OSF-6 antibody|Osi antibody|OSIL antibody|Oxidative stress induced like antibody|p60 antibody|p62 antibody|p62B antibody|Paget disease of bone 3 antibody|PDB 3 antibody|PDB3 antibody|Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody| Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody|Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody|PKC-zeta-interacting protein antibody|Protein kinase C-zeta-interacting protein antibody|Sequestosome 1 antibody| Sequestosome-1 antibody|SQSTM 1 antibody|SQSTM_HUMAN antibody|Sqstm1 antibody|STAP antibody|STONE14 antibody|Ubiquitin binding protein p62 antibody|Ubiquitin-binding protein p62 antibody|ZIP 3 antibody|ZIP antibody|ZIP3 antibody - 基因ID
- 8878
- UniProt
- Q13501
- 途径
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy
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