Recombinant p53 抗体 (full length)
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北京 101111
Quick Overview for Recombinant p53 抗体 (full length) (ABIN7470654)
抗原
See all p53 (TP53) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- full length
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原理
- p53 antibody (053R)
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免疫原
- Recombinant human full-length TP53 protein
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Tonsil: More than 20 % of germinal centre B-cells must show a weak to moderate nuclear staining.
Product Description: p53 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: Luminal epithelial cells must remain p53 negative.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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有效期
- 24 months
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- p53 (TP53) (Tumor Protein P53 (TP53))
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别名
- p53
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背景
- Gene Alias: Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53
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UniProt
- P04637
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途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
抗原
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