Recombinant Collagen IV 抗体
Quick Overview for Recombinant Collagen IV 抗体 (ABIN7477425)
抗原
See all Collagen IV (COL4) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Collagen IV antibody (704R)
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免疫原
- Purified human placental extract
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.
Product Description/Background: Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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- Collagen IV (COL4) (Collagen, Type IV (COL4))
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别名
- Collagen IV
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背景
- Gene Alias: Arresten, BSVD, COL4A1, COL4A1 NC1 domain, COL4A2, COL4A3, COL4A4, COL4A5, collagen alpha-1(IV) chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, Collagen Type IV Alpha 3, Collagen Type IV Alpha 4, Collagen Type IV Alpha 5, RATOR
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UniProt
- P02462, P08572, P29400, P53420
抗原
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