RAD51 抗体 (AA 1-338)
Quick Overview for RAD51 抗体 (AA 1-338) (ABIN7468272)
抗原
See all RAD51 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 1-338
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交叉反应
- 小鸡, 人, 小鼠, 大鼠
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Full length (amino acids 1-338) Rad51 expressed in E. coli.
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亚型
- IgG2b
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 Validation: KO/KD, Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1XPBS ( pH 7), 20 % Glycerol, No Preservative
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- RAD51 (DNA Repair Protein Homolog 1 (RAD51))
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别名
- RAD51 recombinase
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背景
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Synonyms: RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA
Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
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分子量
- 37 kDa
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基因ID
- 5888
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UniProt
- Q06609
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途径
- DNA Damage Repair
抗原
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