SQSTM1 抗体 (pSer403)
Quick Overview for SQSTM1 抗体 (pSer403) (ABIN7464605)
抗原
See all SQSTM1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer403
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Carrier-protein conjugated synthetic peptide surrounding phospho Ser403 of human SQSTM1 / P62. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: Huh7 (5 μM MG132) , Huh7 (10 μM MG132) , HepG2 (3μM Thapsigargin treatment for 12hr and 24hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr)
Validation: Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.08 mg/mL
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缓冲液
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- SQSTM1 (Sequestosome 1 (SQSTM1))
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别名
- sequestosome 1
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背景
- Sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B,This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
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分子量
- 48 kDa
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基因ID
- 8878
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UniProt
- Q13501
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途径
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy
抗原
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