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SQSTM1 抗体

The 小鼠 单克隆 anti-SQSTM1 antibody is suitable to detect SQSTM1 in samples from 人. It has been validated for WB, IF, ICC, FACS 和 IHC (fro).
产品编号 ABIN7464604
发货至: 中国
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Quick Overview for SQSTM1 抗体 (ABIN7464604)

抗原

See all SQSTM1 抗体
SQSTM1 (Sequestosome 1 (SQSTM1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This SQSTM1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

质量等级

KO Validated

克隆位点

GT1478
  • 交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Affinity purified by Protein G.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.

    亚型

    IgG2b
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr) , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr) , HepG2

    Validation: Comparison, KO/KD, Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1XPBS pH 7, 20 % Glycerol, No Preservative

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    SQSTM1 (Sequestosome 1 (SQSTM1))

    别名

    sequestosome 1

    背景

    Sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B,This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]

    分子量

    48 kDa

    基因ID

    8878

    UniProt

    Q13501

    途径

    NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy
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