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Caspase 9 抗体 (Cleaved-Asp330)

This 兔 多克隆 anti-Caspase 9 antibody specifically detects Caspase 9 in WB, ELISA 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN7225380
发货至: 中国
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Quick Overview for Caspase 9 抗体 (Cleaved-Asp330) (ABIN7225380)

抗原

See all Caspase 9 (CASP9) 抗体
Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

适用

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多克隆

标记

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This Caspase 9 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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    AA 250-330, Cleaved-Asp330

    原理

    Cleaved-Caspase-9 (D330) Polyclonal Antibody

    特异性

    Cleaved-Caspase-9 p10 (D330) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 p10 protein resulting from cleavage adjacent to D330.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    免疫原

    Synthesized peptide derived from the C-terminal region of human Caspase-9 p10 at AA range: 250-330

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

    说明

    Primary Antibody

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • 抗原

    Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

    别名

    Caspase-9

    背景

    Rabbit Anti-Cleaved-Caspase-9 (D330) Polyclonal Antibody,CASP9, MCH6, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6,CASP9 (caspase 9) encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1, this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.,Caspase-9

    分子量

    observerd band 17+37kDa

    基因ID

    842

    UniProt

    P55211

    途径

    MAPK Pathway, RTK signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity
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