Recombinant PAK4/PAK5/PAK6 (pSer474), (pSer560), (pSer602) 抗体
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- 抗原
- PAK4/PAK5/PAK6
- 抗体类型
- Recombinant Antibody
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抗原表位
- pSer474, pSer560, pSer602
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 交叉反应
- 人
- 纯化方法
- Affinity-chromatography
- 免疫原
- A synthesized peptide derived from human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602)
- 克隆位点
- 2D1
- 亚型
- IgG
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anti-PAK4/PAK5/PAK6 (pSer474), (pSer560), (pSer602) antibody
适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200,
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- 抗原
- PAK4/PAK5/PAK6
- 背景
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Background: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Aliases: Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4, PAK4, KIAA1142
- UniProt
- O96013, Q9P286, Q9NQU5
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