ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
特异性
At least two isoforms of SLC35D2 are known to exist, this antibody will recognize both isoforms. SLC35D2 antibody is predicted to not cross-react with SLC35D1 or SLC35D3.
纯化方法
SLC35D2 Antibody is affinity chromatography purified via peptide column.
免疫原
SLC35D2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human SLC35D2. The immunogen is located within the last 50 amino acids of SLC35D2.
SLC35D2
适用: 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 594
应用备注
SLC35D2 antibody can be used for detection of SLC35D2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
SLC35D2 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
SLC35D2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
Solute Carrier Family 35 (UDP-GlcNAc/UDP-Glucose Transporter), Member D2 (SLC35D2)
5730408I21Rik antibody, SQV7L antibody, UGTrel8 antibody, hfrc antibody, mUGTrel8 antibody, solute carrier family 35, member D2 antibody, Slc35d2 antibody
背景
SLC35D2 Antibody: The solute carrier family SLC35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The role of the ER-resident SLC family member SLC35D2 is to transport both UDP-glucuronic acid and UDP-N-acetylgalactosamine. Its overexpression in transfected cells modulated cell surface heparin sulfate expression, suggesting that SLC35D2 is involved in heparin sulfate synthesis. SLC35D2-overexpressing cells also showed increased constitutive and hypotonic stress-stimulated release of UDP-GlcNAc, suggesting that SLC35D2 may be involved in UDP-sugar release and cell signaling.