Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
特异性
At least three isoforms are known to exist, this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members.
纯化方法
ZIMP10 Antibody is affinity chromatography purified via peptide column.
免疫原
ZIMP10 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZIMP10. The immunogen is located within amino acids 90 - 140 of ZIMP10.
ZMIZ1
适用: 人, 小鼠, 大鼠
WB, FACS, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 555
应用备注
ZIMP10 antibody can be used for detection of ZIMP10 by Western blot at 0.5 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
ZIMP10 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
ZIMP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ZIMP10 Antibody: ZIMP10, also known as ZMIZ1, is a novel PIAS (protein inhibitor of activated signal transducer and activator of transcription)-like protein initially identified as a transcriptional co-activator of the androgen receptor (AR). ZIMP10 and the related protein ZIMP7 interact with PIAS3 and enhances AR-mediated transcription. Later experiments showed that ZIMP10 is also a co-activator of the p53 tumor suppressor. Mice deficient in ZIMP10 result in embryonic lethality by E10.5, these embryos reveal severe defects in the reorganization of the yolk sac vascular plexus, indicating that ZIMP10 plays an important role in proper vascular development.