Histone 3 抗体 (2meArg8 (asymetric))
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- 抗原 See all Histone 3 (H3) 抗体
- Histone 3 (H3)
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抗原表位
- 2meArg8 (asymetric)
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Histone 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)
- 产品特性
- Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation. Histone H3R8me2a (asymmetric) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
- 纯化方法
- Affinity Purified
- 免疫原
- This Histone H3 dimethyl Arg8 asymmetric antibody was raised against a peptide containing dimethyl-Arg8 (asymmetric) of human histone H3.
- 亚型
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 缓冲液
- Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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