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Histone 3 抗体 (H3K27me)

Name: Histone 3 抗体 (H3K27me)
Brand: antibodies-online
SKU: ABIN3023269
Availability: OutOfStock

H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

Western Blotting (WB) image for anti-Histone 3 (H3) (H3K27me) antibody (ABIN3023269)

Immunohistochemistry of paraffin-embedded human breast cancer using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at dilution of 1:100 (40x lens).

IHC

Immunohistochemistry of paraffin-embedded human rectal cancer tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens).

IHC

Immunohistochemistry (IHC) image for anti-Histone 3 (H3) (H3K27me) antibody (ABIN3023269)

IHC

ChIP

Immunofluorescence analysis of 293T cell using H3K27me3 antibody.

Dot Blot (DB) image for anti-Histone 3 (H3) (H3K27me) antibody (ABIN3023269)

Immunohistochemistry of paraffin-embedded rat brain tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens).

IHC

Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens).

IHC

NNMT regulates intracellular methylation potential and CD44 transcription by inhibiting histone H3K27 methylation.

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at 1:1000 dilution.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521).

Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at dilution of 1:100 (40x lens).

IHC

Chromatin immunoprecipitation analysis of extracts of HeLa, cells, using TriMethyl-Histone H3-K27 Rabbit pAb antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) and rabbit IgG.

ChIP

Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K27 Rabbit pAb (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at dilution of 1:25 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at dilution of 1:100 (40x lens).

IHC

17 images

(14 references)

产品编号 ABIN3023269

发货至: 中国

Datasheet as PDF

抗原 See all Histone 3 (H3) 抗体

Histone 3 (H3)
抗原表位
59

47

45

42

41

37

36

35

35

35

34

32

31

29

29

29

26

25

23

23

23

22

20

18

17

17

17

16

16

16

16

16

15

14

14

13

13

12

12

12

12

12

11

11

10

10

10

9

9

8
H3K27me
适用
1399

941

861

59

42

41

41

40

39

35

35

30

29

22

19

6

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
人
宿主
1217

243

8

4
兔
Compatible Secondaries
克隆类型
1120

351

1
多克隆
标记
821

81

63

63

63

62

62

62

37

33

23

17

17

17

17

17

14

2

1
This Histone 3 antibody is un-conjugated
应用范围
1058

411

378

312

251

188

181

178

163

161

142

126

119

48

38

34

32

20

16

14

7

7

6

4

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

交叉反应

人, 小鼠, 大鼠

产品特性

Methylated Antibodies

纯化方法

Affinity purification

免疫原

A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

亚型

IgG

Top Product

Discover our top product H3 Primary Antibody
anti-Histone 3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone 3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K9me2) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200,ChIP,1:20 - 1:100,ChIP-seq,1:20 - 1:100

限制

仅限研究用
状态

Liquid

缓冲液

PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze / thaw cycles

储存条件

-20 °C

储存方法

Store at -20°C. Avoid freeze / thaw cycles.
Wang, Wang, Lin, Ye, Li, Tu, Shen, Li, Yang, Zhang: "Evolutionary dynamics of 3D genome architecture following polyploidization in cotton." in: Nature plants, Vol. 4, Issue 2, pp. 90-97, (2018) (PubMed).

Cheng, Tan, Lu, Liu, Li, Yuan, Zhao, Zhou: "WOX11 recruits a histone H3K27me3 demethylase to promote gene expression during shoot development in rice." in: Nucleic acids research, Vol. 46, Issue 5, pp. 2356-2369, (2018) (PubMed).

Wu, Tian, Zhang, Tong, Huang, Li, Zhao, Tang, Yuan, Wang, Fang, Gao, Hu, Li, Qin, Yao, Chen, Chen, Zhang, Liu, Sun, Chen, Wong, Ge, Chen, Ji: "In vivo CRISPR screening unveils histone demethylase UTX as an important epigenetic regulator in lung tumorigenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 17, pp. E3978-E3986, (2018) (PubMed).

Dumasia, Kumar, Deshpande, Balasinor: "Estrogen, through estrogen receptor 1, regulates histone modifications and chromatin remodeling during spermatogenesis in adult rats." in: Epigenetics, Vol. 12, Issue 11, pp. 953-963, (2018) (PubMed).

Yu, Liu, Liu, Wang, Liu, Miao, Du, Yang: "Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes." in: Scientific reports, Vol. 8, Issue 1, pp. 6132, (2018) (PubMed).

Song, Li, Wang, Du, Yang: "DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes." in: Scientific reports, Vol. 7, Issue 1, pp. 1687, (2018) (PubMed).

Tang, Guo, Zhu, Huang, Liu, Cai, Yu, Wang: "Metformin inhibits ovarian cancer via decreasing H3K27 trimethylation." in: International journal of oncology, Vol. 52, Issue 6, pp. 1899-1911, (2018) (PubMed).

Cao, Liu, Yue, Liu, Pei, Gu, Wang, Jia: "Iron chelation inhibits cancer cell growth and modulates global histone methylation status in colorectal cancer." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 31, Issue 5, pp. 797-805, (2018) (PubMed).

Wang, Tu, Lin, Lin, Wang, Yang, Ye, Shen, Li, Zhang, Zhou, Nie, Li, Guo, Ma, Huang, Jin, Zhu, Yang, Min, Yuan, Zhang, Lindsey, Zhang: "Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication." in: Nature genetics, Vol. 49, Issue 4, pp. 579-587, (2017) (PubMed).

Zhou, Liu, Zhou, Zhou, Zhao, Li, Zhou: "Cooperation between the H3K27me3 Chromatin Mark and Non-CG Methylation in Epigenetic Regulation." in: Plant physiology, Vol. 172, Issue 2, pp. 1131-1141, (2017) (PubMed).

Kumar, Dumasia, Deshpande, Balasinor: "Direct regulation of genes involved in sperm release by estrogen and androgen through their receptors and coregulators." in: The Journal of steroid biochemistry and molecular biology, Vol. 171, pp. 66-74, (2017) (PubMed).

Su, Li, Lei, Wang, Zhao, Cai, Wang, Li, Wu: "The EZH1-SUZ12 complex positively regulates the transcription of NF-κB target genes through interaction with UXT." in: Journal of cell science, Vol. 129, Issue 12, pp. 2343-53, (2017) (PubMed).

Yi, Guo, Guo, Sun, Yang, Wang, Li, Xie, Cai, Wang: "EZH2-mediated epigenetic silencing of TIMP2 promotes ovarian cancer migration and invasion." in: Scientific reports, Vol. 7, Issue 1, pp. 3568, (2017) (PubMed).

Liu, Zhou, Wang, Ye, Zhao, Xu, Zhou, Tan, Cheng, Zhou: "Regulation of histone methylation and reprogramming of gene expression in the rice inflorescence meristem." in: The Plant cell, Vol. 27, Issue 5, pp. 1428-44, (2016) (PubMed).
抗原

Histone 3 (H3)

别名

Histone H3 (H3 产品)

别名

H-3 antibody, histocompatibility 3 antibody, H3 antibody

背景

Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,H3.4,H3/g,H3FT,H3t,HIST3H3,Histone H3,HIST1H3A,Signal Transduction,MAPK-Erk Signaling Pathway,MAPK-P38 Signaling Pathway,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Methylation,Histone H3

分子量

15 kDa

基因ID

8290

UniProt

Q16695