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Histone 3 抗体 (acLys9)

Name: Histone 3 抗体 (acLys9)
Brand: antibodies-online
SKU: ABIN6972014
Availability: OutOfStock

This anti-Histone 3 antibody is a 兔多克隆 antibody detecting Histone 3 in WB, IF, ChIP, DB, ICC, ChIP-seq 和 CUT&Tag. Suitable for 人和小鼠. This Primary Antibody has been cited in 1 publication.

Western blot of Histone H3K9ac antibody (pAb). (Histone 3 抗体 (acLys9))

Immunofluorescence stain of Histone H3K9ac antibody (pAb). (Histone 3 抗体 (acLys9))

ChIP-seq

ChIP

Histone H3K9ac antibody (pAb) tested by dot blot analysis. (Histone 3 抗体 (acLys9))

5 images

(1 reference)

产品编号 ABIN6972014

发货至: 中国

Datasheet as PDF

Quick Overview for Histone 3 抗体 (acLys9) (ABIN6972014)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

1344
908
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人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

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35
22
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1
1
1
1

Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Discover our top product H3 Primary Antibody

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acLys9

产品特性

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys9 can also be mono-, di- or trimethylated. The methylation of this residue is often associated with transcriptional repression. However, acetylation of histone H3 Lys9 is associated with transcriptional activation of the genes. Histone H3K9ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, CUT&Tag, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.

纯化方法

Protein A Chromatography

免疫原

This Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of histone H3.

亚型

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

Recommended antibody dilutions for different applications:
ChIP: 10 µg per ChIP
ChIP-Seq: 3 µg each
ICC/IF: 2 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution
CUT&Tag: 1 µg per 50 µl reaction
Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

1 μg/μL

缓冲液

Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Kee, Thudium, Renner, Glastad, Palozola, Zhang, Li, Lan, Cesare, Poleshko, Kiseleva, Truitt, Cardenas-Diaz, Zhang, Xie, Kotton, Alysandratos, Epstein, Shi et al.: "SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry." in: Nature, (2022) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

分子量

17 kDa

NCBI登录号

NP_003522