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Histone 3 抗体 (H3K4me3)

Name: Histone 3 抗体 (H3K4me3)
SKU: ABIN2668472
Availability: OutOfStock

Cited in 75+ publications. The 兔多克隆 anti-Histone 3 antibody (ABIN2668472) specifically detects Histone 3 in WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 和 CUT&RUN. The antibody is reactive with 人, 小鼠和 Saccharomyces cerevisiae samples.

Histone H3 trimethyl Lys4 antibody tested by Western blot. (Histone 3 抗体 (H3K4me3))

Histone H3 trimethyl Lys4 antibody tested by immunofluorescence. (Histone 3 抗体 (H3K4me3))

ChIP

Histone H3 trimethyl Lys4 antibody tested by dot blot analysis. (Histone 3 抗体 (H3K4me3))

ChIP-seq

5 images

(75 references)

产品编号 ABIN2668472

发货至: 中国

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中国
北京 101111

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Beijing Economic Technological Development Area

Room 801-803

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Quick Overview for Histone 3 抗体 (H3K4me3) (ABIN2668472)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

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人, 小鼠, Saccharomyces cerevisiae

宿主

兔

克隆类型

多克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Discover our top product H3 Primary Antibody

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H3K4me3

原理

Histone H3K4me3 antibody (pAb)

纯化方法

Unpurified

免疫原

This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3.
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP: 3 - 5 µL per ChIP ChIP-Seq: 3 µL each ICC/IF: 1:500 - 1:1,000 dilution WB: 1:500 - 1:2,000 dilution CUT&Tag: 1 µL per 50 µL reaction* CUT&RUN: 1 µL per 50 µL reaction TIP-ChIP: 2 µL *This antibody has been validated for CUT&Tag using Active Motif's CUT&Tag-IT Assay Kit, Catalog No. 53160. modENCODE validation: this antibody was validated for ChIP-Seq in this study (see reference). NGS-QC certification: this antibody has been processed by the NGS-QC generator. For additional details, click here.. **Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Henikoff, Henikoff, Kaya-Okur, Ahmad: "Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation." in: eLife, Vol. 9, (2020) (PubMed).

Brahma, Henikoff: "RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast." in: Molecular cell, Vol. 73, Issue 2, pp. 238-249.e3, (2019) (PubMed).

Pan, Guan, Liu, Li, Wang, Wu, Zhou, Zhang, Ren, Zhang, Li, Yang, Hao, Yuan, Yuan, Wang, Ju, Mao, Li, Qu, Tang, Liu: "SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2." in: Cell research, Vol. 26, Issue 2, pp. 190-205, (2016) (PubMed).

Toth, Papp, Brulois, Choi, Gao, Jung: "LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection." in: PLoS pathogens, Vol. 12, Issue 9, pp. e1005878, (2016) (PubMed).

Lowe, Gemma, Rakyan, Holland: "Sexually dimorphic gene expression emerges with embryonic genome activation and is dynamic throughout development." in: BMC genomics, Vol. 16, pp. 295, (2015) (PubMed).

Weirich, Kudithipudi, Kycia, Jeltsch: "Somatic cancer mutations in the MLL3-SET domain alter the catalytic properties of the enzyme." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 36, (2015) (PubMed).

Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

Brown, Esterhazy, Sarde, London, Pullabhatla, Osma-Garcia, Al-Bader, Ortiz, Elgueta, Arno, de Rinaldis, Mucida, Lord, Noelle: "Retinoic acid is essential for Th1 cell lineage stability and prevents transition to a Th17 cell program." in: Immunity, Vol. 42, Issue 3, pp. 499-511, (2015) (PubMed).

Sabò, Kress, Pelizzola, de Pretis, Gorski, Tesi, Morelli, Bora, Doni, Verrecchia, Tonelli, Fagà, Bianchi, Ronchi, Low, Müller, Guccione, Campaner, Amati: "Selective transcriptional regulation by Myc in cellular growth control and lymphomagenesis." in: Nature, Vol. 511, Issue 7510, pp. 488-92, (2014) (PubMed).

Fereres, Simón, Mohd-Sarip, Verrijzer, Busturia: "dRYBP counteracts chromatin-dependent activation and repression of transcription." in: PLoS ONE, Vol. 9, Issue 11, pp. e113255, (2014) (PubMed).

Putiri, Tiedemann, Liu, Choi, Robertson: "Impact of human MLL/COMPASS and polycomb complexes on the DNA methylome." in: Oncotarget, Vol. 5, Issue 15, pp. 6338-52, (2014) (PubMed).

Dhimolea, Wadia, Murray, Settles, Treitman, Sonnenschein, Shioda, Soto: "Prenatal exposure to BPA alters the epigenome of the rat mammary gland and increases the propensity to neoplastic development." in: PLoS ONE, Vol. 9, Issue 7, pp. e99800, (2014) (PubMed).

Konsavage, Yochum: "The myc 3' wnt-responsive element suppresses colonic tumorigenesis." in: Molecular and cellular biology, Vol. 34, Issue 9, pp. 1659-69, (2014) (PubMed).

Asmar, Hother, Kulosman, Treppendahl, Nielsen, Ralfkiaer, Pedersen, Møller, Ralfkiaer, de Nully Brown, Grønbæk: "Diffuse large B-cell lymphoma with combined TP53 mutation and MIR34A methylation: Another "double hit" lymphoma with very poor outcome?" in: Oncotarget, Vol. 5, Issue 7, pp. 1912-25, (2014) (PubMed).

DAnselmi, Masiello, Cucina, Proietti, Dinicola, Pasqualato, Ricci, Dobrowolny, Catizone, Palombo, Bizzarri: "Microenvironment promotes tumor cell reprogramming in human breast cancer cell lines." in: PLoS ONE, Vol. 8, Issue 12, pp. e83770, (2014) (PubMed).

Kelso, Baumgart, Eickhoff, Albert, Antrecht, Lemcke, Klebl, Meisterernst: "Cyclin-dependent kinase 7 controls mRNA synthesis by affecting stability of preinitiation complexes, leading to altered gene expression, cell cycle progression, and survival of tumor cells." in: Molecular and cellular biology, Vol. 34, Issue 19, pp. 3675-88, (2014) (PubMed).

Belyea, Xu, Pentz, Medrano, Li, Hu, Turner, Legallo, Jones, Tario, Liang, Gross, Sequeira-Lopez, Gomez: "Identification of renin progenitors in the mouse bone marrow that give rise to B-cell leukaemia." in: Nature communications, Vol. 5, pp. 3273, (2014) (PubMed).

Amatori, Ballarini, Faversani, Belloni, Fusar, Bosari, Pelicci, Minucci, Fanelli: "PAT-ChIP coupled with laser microdissection allows the study of chromatin in selected cell populations from paraffin-embedded patient samples." in: Epigenetics & chromatin, Vol. 7, pp. 18, (2014) (PubMed).

Kumar, Duester: "Retinoic acid controls body axis extension by directly repressing Fgf8 transcription." in: Development (Cambridge, England), Vol. 141, Issue 15, pp. 2972-7, (2014) (PubMed).

Rafehi, Balcerczyk, Lunke, Kaspi, Ziemann, Kn, Okabe, Khurana, Ooi, Khan, Du, Chang, Haviv, Keating, Karagiannis, El-Osta: "Vascular histone deacetylation by pharmacological HDAC inhibition." in: Genome research, Vol. 24, Issue 8, pp. 1271-84, (2014) (PubMed).

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抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

分子量

17 kDa

基因ID

3020

NCBI登录号

NP_003522