Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys9 can also be mono-, di- or trimethylated. The methylation of this residue is often associated with transcriptional repression. However, acetylation of histone H3 Lys9 is associated with transcriptional activation of the genes. Histone H3K9ac antibody (mAb) (Clone 2G1F9) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
纯化方法
Protein G Chromatography
免疫原
This antibody was raised against a peptide containing acetyl-lysine 9 of human Histone H3.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.