Histone 3 抗体 (acLys9)
Quick Overview for Histone 3 抗体 (acLys9) (ABIN6972004)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- acLys9
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产品特性
- Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Acetylation of Lys9 is associated with trascriptional activation of genes. Histone H3K9ac antibody (mAb) (Clone 1B10) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
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纯化方法
- Protein A Chromatography
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免疫原
- The Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human Histone H3.
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亚型
- IgG3
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in 0.1 M Tris, pH 8.0, 1 M NaCl with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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: "SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry." in: Nature, (2022) (PubMed).
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: "SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry." in: Nature, (2022) (PubMed).
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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分子量
- 17
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NCBI登录号
- NP_003522
抗原
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