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Histone 3 抗体 (acLys9)

Cited in 1 publication. This anti-Histone 3 antibody is a 小鼠 单克隆 antibody detecting Histone 3 in WB, IF, ChIP, ICC, DB 和 ChIP-seq. Suitable for 人 和 小鼠.
产品编号 ABIN6972004
发货至: 中国
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Quick Overview for Histone 3 抗体 (acLys9) (ABIN6972004)

抗原

See all Histone 3 (H3) 抗体
Histone 3 (H3) (Histone H3 (H3))

适用

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人, 小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

克隆位点

1B10
  • 抗原表位

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    acLys9

    产品特性

    Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Acetylation of Lys9 is associated with trascriptional activation of genes. Histone H3K9ac antibody (mAb) (Clone 1B10) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.

    纯化方法

    Protein A Chromatography

    免疫原

    The Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human Histone H3.

    亚型

    IgG3
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 缓冲液

    Purified IgG in 0.1 M Tris, pH 8.0, 1 M NaCl with 30 % glycerol and 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Kee, Thudium, Renner, Glastad, Palozola, Zhang, Li, Lan, Cesare, Poleshko, Kiseleva, Truitt, Cardenas-Diaz, Zhang, Xie, Kotton, Alysandratos, Epstein, Shi et al.: "SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry." in: Nature, (2022) (PubMed).

  • 抗原

    Histone 3 (H3) (Histone H3 (H3))

    别名

    Histone H3

    分子量

    17

    NCBI登录号

    NP_003522
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