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Histone 3 抗体 (H3K27ac)

This anti-Histone 3 antibody is a 兔 多克隆 antibody detecting Histone 3 in WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 和 PeA. Suitable for 人.
产品编号 ABIN6952337
发货至: 中国

Quick Overview for Histone 3 抗体 (H3K27ac) (ABIN6952337)

抗原

See all Histone 3 (H3) 抗体
Histone 3 (H3) (Histone H3 (H3))

适用

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宿主

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克隆类型

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多克隆

标记

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This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA)

质量等级

ChIP-seq Grade
  • 抗原表位

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    H3K27ac

    特异性

    Polyclonal antibody raised in rabbit against the region of histone H3 containing the acetylated lysine 27 (H3K27ac), using a KLH-conjugated synthetic peptide.

    交叉反应

    Arabidopsis, 小鼠, 大鼠

    交叉反应 (详细)

    wide range

    纯化方法

    Peptide affinity purified

    免疫原

    synthetic peptide
  • 应用备注

    ChIP/ChIP-seq 1 μg:ChIP
    ELISA 1:500
    Dot Blot 1:20,000
    WB 1:1,000
    IF 1:500

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    2.8 μg/μL

    缓冲液

    PBS, 0.05 % azide, 0.05 % ProClin300

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C
  • 抗原

    Histone 3 (H3) (Histone H3 (H3))

    别名

    Histone 3

    背景

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.

    UniProt

    P68431
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