Smooth Muscle Actin 抗体 (N-Term)
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- 抗原 See all Smooth Muscle Actin (ACTA2) 抗体
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
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抗原表位
- N-Term
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适用
- 人, 大鼠, 小鼠, 小鸡, Cow, 兔, 猴, Baboon, 豚鼠, 犬, Cat, Pig, 山羊, 绵羊
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Smooth Muscle Actin antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- 特异性
- Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
- 克隆位点
- 1A4-same as asm-1
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product ACTA2 Primary Antibody
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anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody
ACTA2 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IF, ELISA, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyVerified ACTA2 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyKO Validated ACTA2 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyACTA2 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 1A4-ACTA2-791 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-124) antibodyACTA2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 2-124) antibodyACTA2 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 5C1H11 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibodyACTA2 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 4A4 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibodyACTA2 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibodyACTA2 适用: 人, 大鼠, 小鼠, 小鸡, 兔, 山羊 WB, IHC, ELISA, IHC (p), ICC, IHC (fro), IEM 宿主: 小鼠 Monoclonal alpha-SM1-1A4 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-377) antibodyACTA2 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 4A8-2H3 unconjugated
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- 应用备注
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Positive Control: HeLa cells (FACS/IF). Blood vessels in all tissues, smooth muscle or leiomyosarcoma (IHC).
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
- 别名
- ACTA2 (ACTA2 产品)
- 别名
- AAT6 antibody, ACTSA antibody, MYMY5 antibody, GAMMA-ACTIN antibody, ACT antibody, ACTA3 antibody, ACTE antibody, ACTL3 antibody, ACTSG antibody, Act-4 antibody, Act4 antibody, SMGA antibody, 0610041G09Rik antibody, Actvs antibody, SMalphaA antibody, a-SMA antibody, alphaSMA antibody, wu:fb63d03 antibody, ASMA antibody, SMactin antibody, a-sma antibody, aat6 antibody, actsa antibody, mymy5 antibody, sm-actin antibody, ACTA2 antibody, actc1 antibody, ACT-4 antibody, asma antibody, ACTGE antibody, actin, alpha 2, smooth muscle, aorta antibody, actin, gamma 2, smooth muscle, enteric antibody, actin, alpha 2, smooth muscle, aorta L homeolog antibody, ACTA2 antibody, ACTG2 antibody, Actg2 antibody, Acta2 antibody, acta2 antibody, acta2.L antibody
- 分子量
- 42kDa
- 基因ID
- 59
- UniProt
- P62736
- 途径
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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