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BAX 抗体 (AA 3-16)

BAX 适用: 人, 猴 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 2D2 unconjugated
产品编号 ABIN6940472
发货至: 中国
  • 抗原 See all BAX 抗体
    BAX (BCL2-Associated X Protein (BAX))
    抗原表位
    • 40
    • 32
    • 19
    • 18
    • 15
    • 11
    • 10
    • 10
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    • 8
    • 8
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    • 3
    • 3
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 3-16
    适用
    • 258
    • 156
    • 143
    • 46
    • 31
    • 26
    • 25
    • 18
    • 17
    • 10
    • 9
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 猴
    宿主
    • 170
    • 96
    • 4
    • 2
    小鼠
    克隆类型
    • 182
    • 90
    单克隆
    标记
    • 143
    • 16
    • 13
    • 13
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This BAX antibody is un-conjugated
    应用范围
    • 242
    • 114
    • 72
    • 65
    • 52
    • 52
    • 52
    • 45
    • 42
    • 14
    • 12
    • 8
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    特异性
    Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
    无交叉反应
    小鼠, 大鼠
    纯化方法
    Purified by Protein A/G
    免疫原
    A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
    克隆位点
    2D2
    亚型
    IgG1 kappa
    Top Product
    Discover our top product BAX Primary Antibody
  • 应用备注

    Positive Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    BAX (BCL2-Associated X Protein (BAX))
    别名
    BAX (BAX 产品)
    分子量
    21kDa
    基因ID
    581
    UniProt
    Q07812
    途径
    p53 Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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