Recombinant Histone H1 抗体
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- 抗原 See all Histone H1 抗体
- Histone H1
- 抗体类型
- Recombinant Antibody
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This Histone H1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Staining Methods (StM)
- 免疫原
- Nuclei of human leukemia biopsy cells
- 克隆位点
- AE-4
- 亚型
- IgG
- Top Product
- Discover our top product Histone H1 Primary Antibody
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anti-Histone H1 antibody
适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal r1415-1 unconjugated Recombinant Antibody
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM, CUT&RUN 宿主: 小鼠 Monoclonal AE-4 unconjugated
anti-Histone H1 (AA 1-105) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 兔 Monoclonal HH1-1784R unconjugated Recombinant Antibody
anti-Histone H1 (acLys52) antibody适用: 人, 小鼠, 大鼠 FACS, IHC (p), ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 150-207) antibody适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (Lys1535) antibody适用: 人, 小鼠 IF, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 1-194) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人 IHC, WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Positive Control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma (IHC).
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Histone H1
- 别名
- Histone H1 (Pan Nuclear Marker) (Histone H1 产品)
- 别名
- H10 antibody, H1FV antibody, H1 histone family member 0 antibody, H1F0 antibody
- 背景
- Please note that this antibody is a recombinant Rabbit version of original anti-histone H1 antibody (Clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a pure preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.
- 分子量
- ~30kDa
- 基因ID
- 3005
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