Histone H1 抗体
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- 抗原 See all Histone H1 抗体
- Histone H1
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Histone H1 antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Nuclei of human leukemia biopsy cells
- 克隆位点
- AE-4
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product Histone H1 Primary Antibody
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anti-Histone H1 antibody
适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal r1415-1 unconjugated Recombinant Antibody
anti-Histone H1 (AA 1-105) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 兔 Monoclonal HH1-1784R unconjugated Recombinant Antibody
anti-Histone H1 (acLys52) antibody适用: 人, 小鼠, 大鼠 FACS, IHC (p), ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 150-207) antibody适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (Lys1535) antibody适用: 人, 小鼠 IF, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 1-194) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (N-Term) antibody适用: 人, 小鼠, 大鼠, Cow, 兔 IF, IHC, WB, IC 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 1-50) antibody适用: 人, 小鼠 IF, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Histone H1
- 别名
- Histone H1 (Pan Nuclear Marker) (Histone H1 产品)
- 别名
- H10 antibody, H1FV antibody, H1 histone family member 0 antibody, H1F0 antibody
- 背景
- Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
- 分子量
- ~30kDa
- 基因ID
- 3005
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