Cytokeratin 7 抗体
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- 抗原 See all Cytokeratin 7 (KRT7) 抗体
- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cytokeratin 7 antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
- 交叉反应 (详细)
- Human. Rat.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human KRT7 protein (KRT7/760), OTN 11, ovarian carcinoma cell line (OV-TL12/30)
- 克隆位点
- KRT7-760-OV-TL12-30
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product KRT7 Primary Antibody
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Carcinoma of ovary, lung, cervix or breast.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
- 别名
- KRT7 (KRT7 产品)
- 别名
- CK7 antibody, K2C7 antibody, K7 antibody, SCL antibody, D15Wsu77e antibody, Krt2-7 antibody, KRT7 antibody, keratin 7 antibody, KRT7 antibody, Krt7 antibody
- 背景
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CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7,Cytokeratin 7 (Glandular and Transitional Epithelial Marker)
Cellular localisation: Cytoplasmic - 分子量
- 55kDa
- 基因ID
- 3855, 411501
- UniProt
- P08729
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