HLA-A 抗体
-
- 抗原 See all HLA-A 抗体
- HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This HLA-A antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10
- 克隆位点
- CATA-1
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product HLA-A Primary Antibody
-
-
- 应用备注
-
Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: THP-1 and Jurkat cells. Tonsil or lymph node.
- 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
-
- 抗原
- HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))
- 别名
- HLA-A (HLA-A 产品)
- 别名
- HLAA antibody, major histocompatibility complex, class I, A antibody, HLA-A antibody
- 背景
-
HLA-A, HLA class 1A, MHC class I HLA-A,HLA-Aw32 & HLA-A25 (MHC I)
Cellular localisation: Cell Surface - 分子量
- ~41kDa
- 基因ID
- 3105, 181244, 654404
- UniProt
- P01889, P30443, P30499
- 途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
-