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GAPDH 抗体

GAPDH 适用: 人, 小鼠, 大鼠, Pig, Cow, 哺乳动物 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal 1D4 unconjugated
产品编号 ABIN1580424
发货至: 中国
  • 抗原 See all GAPDH 抗体
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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    人, 小鼠, 大鼠, Pig, Cow, 哺乳动物
    宿主
    • 154
    • 89
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    小鼠
    克隆类型
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    单克隆
    标记
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    This GAPDH antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    纯化方法
    conc. tissue culture supernatant
    克隆位点
    1D4
    亚型
    IgM
    Top Product
    Discover our top product GAPDH Primary Antibody
  • 应用备注
    The antibody solution can be used at dilutions of at least 1:100 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000 or lower. It is very useful as a loading control for quantitative western blotting.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Supplied as purified antibody at 1mg/mL in PBS and glycerol (1:1) with 5mM NaN3
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store at 4°C short term or -20°C long term.
  • 抗原
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    别名
    Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH 产品)
    别名
    G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody
    背景
    Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. The HGNC name for this protein is GAPDH.
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