GAPDH 抗体 (AA 136-335)
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北京 101111
Quick Overview for GAPDH 抗体 (AA 136-335) (ABIN5518757)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 136-335
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原理
- Anti-GAPDH Antibody
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特异性
- No cross reactivity with other proteins.
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交叉反应 （详细）
- No cross-reactivity with other proteins
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预测反应
- Human GAPDH shares 95%,94.5% amino acid (aa) sequence identity with mouse,rat GAPDH,respectively.
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产品特性
- Anti-GAPDH Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human GAPDH recombinant protein (Position: N136-E335). Human GAPDH shares 95% and 94.5% amino acid (aa) sequence identity with mouse and rat GAPDH, respectively.
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亚型
- IgG
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, ICC, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibodyGAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibodyVerified GAPDH 适用: 人, 大鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibodyGAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibodyGAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibodyVerified GAPDH 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 233-259), (C-Term) antibodyGAPDH 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16543 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibodyGAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibodyGAPDH 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibodyGAPDH 适用: 人 WB, IHC, ICC, IP, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat, Chicken, Zebrafish Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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: "TGEV infection up-regulates FcRn expression via activation of NF-κB signaling." in: Scientific reports, Vol. 6, pp. 32154, (2018) (PubMed).
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: "Evaluation of the circulating level of fibroblast activation protein α for diagnosis of esophageal squamous cell carcinoma." in: Oncotarget, Vol. 8, Issue 18, pp. 30050-30062, (2018) (PubMed).
: "Glucagon-Like Peptide-1 (GLP-1) Treatment Ameliorates Cognitive Impairment by Attenuating Arc Expression in Type 2 Diabetic Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 4334-4342, (2018) (PubMed).
: "Iguratimod prevents ovariectomy‑induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator‑activated receptor‑γ." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8200-8208, (2018) (PubMed).
: "Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8953-8963, (2018) (PubMed).
: "Autophagy promotes fibrosis and apoptosis in the peritoneum during long-term peritoneal dialysis." in: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 1190-1201, (2018) (PubMed).
: "Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 9257967, (2018) (PubMed).
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: "Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes." in: Inflammation, Vol. 41, Issue 2, pp. 562-568, (2018) (PubMed).
: "Genomic correlates of response to immune checkpoint therapies in clear cell renal cell carcinoma. ..." in: Science (New York, N.Y.), Vol. 359, Issue 6377, pp. 801-806, (2018) (PubMed).
: "MALAT1 enhanced the proliferation of human osteoblasts treated with ultra‑high molecular weight polyethylene by targeting VEGF via miR‑22‑5p." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1536-1546, (2018) (PubMed).
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- GAPDH
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背景
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Background: Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37 kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus.
Gene Full Name: glyceraldehyde-3-phosphate dehydrogenase
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分子量
- 36 kDa
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基因ID
- 2597
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UniProt
- P04406
抗原
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