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GAPDH 抗体 (C-Term)

Name: GAPDH 抗体 (C-Term)
SKU: ABIN392354
Availability: OutOfStock

GAPDH 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB16543 unconjugated

Western blot analysis of GDH Antibody (C-term ) 7873b in , , CEM cell line lysates (35 μg/lane). (GAPDH 抗体 (C-Term))

Flow cytometric analysis of HepG2 cells using GDH Antibody (C-term )(bottom histogram) compared to a negative control cell (top histogram). (GAPDH 抗体 (C-Term))

FACS

Confocal immunofluorescent analysis of GDH Antibody (C-term ) 7873b with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (GAPDH 抗体 (C-Term))

All lanes : Anti-GDH Antibody (C-term ) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (GAPDH 抗体 (C-Term))

Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GDH Antibody (C-term ) 7873b. (GAPDH 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human prostate carcinoma with GDH Antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (GAPDH 抗体 (C-Term))

IHC (p)

7 images

(1 reference)

产品编号 ABIN392354

发货至: 中国

Datasheet as PDF

抗原 See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
抗原表位
36

21

13

9

7

6

6

5

4

4

3

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 233-259, C-Term
适用
211

142

135

75

65

55

36

28

23

21

20

19

18

16

12

12

11

10

9

8

6

4

4

4

4

3

3

3

3

3

2

2

1

1

1

1

1
人
宿主
158

97

17

10

4
兔
Compatible Secondaries
克隆类型
179

105
多克隆
标记
155

35

22

16

5

5

5

5

4

4

4

3

3

3

3

3

2

2

2

1

1

1

1

1
This GAPDH antibody is un-conjugated
应用范围
233

98

72

70

45

40

35

30

16

16

15

15

8

6

5

4

4

3

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

预测反应

C, M, Pig, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-259 amino acids from the C-terminal region of human GAPDH.

克隆位点

RB16543

亚型

Ig Fraction

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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠, 兔, Pig, 小鸡, Cow, Various Species WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Wang, Chen, Wang, Yu, Zhao, Zhang, Tang, Peng: "Metalloproteases meprin-ɑ (MEP1A) is a prognostic biomarker and promotes proliferation and invasion of colorectal cancer." in: BMC cancer, Vol. 16, pp. 383, (2017) (PubMed).
抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

GAPDH (GAPDH 产品)

别名

G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

背景

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

分子量

36053

基因ID

2597

NCBI登录号

NP_001243728, NP_002037

UniProt

P04406