ATP6V0A2 抗体 (AA 72-121)
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- 抗原 See all ATP6V0A2 抗体
- ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))
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抗原表位
- AA 72-121
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ATP6V0A2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ATP6V0A2
- 预测反应
- Percent identity by BLAST analysis: Human, Rat (100%) Dog, Horse (92%) Pig (91%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa72-121 of human ATP6V0A2 (Q9Y487, NP_036595). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat (100%), Monkey, Galago, Elephant, Dog, Bat, Horse (92%), Pig (91%), Bovine, Platypus (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product ATP6V0A2 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled Water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))
- 别名
- ATP6V0A2 (ATP6V0A2 产品)
- 别名
- A2 antibody, ARCL antibody, ARCL2A antibody, ATP6A2 antibody, ATP6N1D antibody, J6B7 antibody, RTF antibody, STV1 antibody, TJ6 antibody, TJ6M antibody, TJ6S antibody, VPH1 antibody, WSS antibody, 8430408C20Rik antibody, AI385560 antibody, ATP6a2 antibody, AW489264 antibody, Atp6n1d antibody, Atp6n2 antibody, C76904 antibody, ISF antibody, SHIF antibody, Stv1 antibody, TJ6s antibody, Tj6 antibody, V-ATPase 116 kDa antibody, V-ATPase a2 antibody, Cc1-3 antibody, J6b7 antibody, atp6v0a2 antibody, si:ch211-199i18.4 antibody, si:ch211-106a19.2 antibody, ATPase H+ transporting V0 subunit a2 antibody, ATPase, H+ transporting, lysosomal V0 subunit A2 antibody, ATPase, H+ transporting, lysosomal V0 subunit a2a antibody, ATPase, H+ transporting, lysosomal V0 subunit a2b antibody, ATP6V0A2 antibody, Atp6v0a2 antibody, atp6v0a2a antibody, atp6v0a2b antibody
- 背景
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Name/Gene ID: ATP6V0A2
Subfamily: ATPase - V type
Family: Transporter
Synonyms: ATP6V0A2, A2V-ATPase, ARCL, ARCL2A, ATP6A2, ATP6N1D, RTF, STV1, V-ATPase 116 kDa, TJ6S, WSS, J6B7, TJ6, TJ6M, V-ATPase 116 kDa isoform a2, VPH1 - 基因ID
- 23545
- NCBI登录号
- NP_036595
- UniProt
- Q9Y487
- 途径
- Transition Metal Ion Homeostasis, Proton Transport
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