RAD51 抗体
Quick Overview for RAD51 抗体 (ABIN6719396)
抗原
See all RAD51 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-Rad51 Antibody Picoband®
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序列
- KKLEEAGFHT VEAVAYAPKK ELINIKGISE AKADK
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Rad51 Antibody Picoband® (ABIN6719396). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Rad51, which shares 97.1% amino acid (aa) sequence identity with both mouse and rat Rad51.
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亚型
- IgG
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anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-339) antibody
RAD51 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-DNA Repair Protein Homolog 1 (RAD51) (Center) antibodyRAD51 适用: 人, 小鼠 WB, IP, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Homolog 1 (RAD51) (AA 81-180) antibodyRAD51 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-228) antibodyRAD51 适用: 人 WB, ELISA, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-339) antibodyRAD51 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-242) antibodyRAD51 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E5-E5 unconjugated
anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-134) antibodyRAD51 适用: 人 IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal RAD51-2702 unconjugated
anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-134) antibodyRAD51 适用: 人 WB, IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal RAD51-2701 unconjugated
anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-258) antibodyRAD51 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-242) antibodyRAD51 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 4C11-B4 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells1. Kato, M., Yano, K., Matsuo, F., Saito, H., Katagiri, T., Kurumizaka, H., Yoshimoto, M., Kasumi, F., Akiyama, F., Sakamoto, G., Nagawa, H., Nakamura, Y., Miki, Y. : Identification of Rad51 alteration in patients with bilateral breast cancer. J. Hum. Genet. 45: 133-137, 2000. 2. Daniel DC (2002). "Highlight: BRCA1 and BRCA2 proteins in breast cancer.". Microsc. Res. Tech. 59 (1): 68-83. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- RAD51 (DNA Repair Protein Homolog 1 (RAD51))
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别名
- RAD51
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背景
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Synonyms: DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A, RAD51, RAD51A, RECA
Tissue Specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Background: DNA repair protein RAD51 homolog 1, also known as RAD51A, is a human gene. The Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair. BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. RAD51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.
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分子量
- 39 kDa
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基因ID
- 5888
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UniProt
- Q06609
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途径
- DNA Damage Repair
抗原
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