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- 抗原 See all CD45.1 products
- CD45.1 (CD45 (CD45.1 Allogen))
- 适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD45.1 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity with CD45.1 from other sources has not been tested
- 纯化方法
- Fluorescein conjugated CD45.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45.1. . Anti-CD45.1 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
- 免疫原
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Immunogen: Anti-CD45.1 Antibody (Monoclonal) was produced by repeated immunizations with CD45.1 antigen.
- 克隆位点
- A20
- 亚型
- IgG2a
- Labeling Ratio
- 2-8
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anti-CD45 (CD45.1 Allogen) antibody (Cy7,PE)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 Cy7,PE
anti-CD45 (CD45.1 Allogen) antibody (violetFluor™ 450)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 violetFluor™ 450
anti-CD45 (CD45.1 Allogen) antibody (PerCP-Cy5.5)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 PerCP-Cy5.5
anti-CD45 (CD45.1 Allogen) antibody (PE)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 PE
anti-CD45 (CD45.1 Allogen) antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 PE-Cy7
anti-CD45 (CD45.1 Allogen) antibody适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 unconjugated
anti-CD45 (CD45.1 Allogen) antibody (APC)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 APC
anti-CD45 (CD45.1 Allogen) antibody (Biotin)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 Biotin
anti-CD45 (CD45.1 Allogen) antibody (APC-Cy7)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 APC-Cy7
anti-CD45 (CD45.1 Allogen) antibody (FITC)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 FITC
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-CD45.1 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
Immunoprecipitation Dilution: User Optimized
Flow Cytometry Dilution: 10 μL/10^6 cells (0.1 μg)
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
0.09 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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- 抗原
- CD45.1 (CD45 (CD45.1 Allogen))
- 别名
- CD45.1 (CD45.1 产品)
- 背景
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Synonyms: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5
Background: The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.
Gene Name: Ptprc
- UniProt
- P06800
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