CENPF 抗体 (AA 1759-2093)
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Quick Overview for CENPF 抗体 (AA 1759-2093) (ABIN189989)
抗原
See all CENPF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1759-2093
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原理
- Anti-Mitosin Mouse Monoclonal Antibody
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特异性
- This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
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交叉反应
- 人
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免疫原
- GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.
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亚型
- IgG2a
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anti-Centromere Protein F (CENPF) (AA 2350-2400) antibody
CENPF 适用: 人 IHC, IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (C-Term) antibodyCENPF 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 436-637) antibodyCENPF 适用: 人 ELISA, WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (C-Term) antibodyCENPF 适用: 人, 小鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 1800-1850) antibodyCENPF 适用: 人 IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 1759-2093) antibodyCENPF 适用: 人 IHC, WB, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 551-650) antibodyCENPF 适用: 人 ELISA, WB, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (C-Term) antibodyVerified CENPF 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 1800-1850) antibodyCENPF 适用: 人 WB, IP, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 2350-2400) antibodyCENPF 适用: 人 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoblotting: use at 1-10 μg/mL.
Immunoprecipitation: use at 1-10 μg/mL.
Immunohistochemistry: use at 10 μg/mL with frozen or paraffin-embedded sections.
Positive controls: Human tissue containing rapidly proliferating cells, such as lymphoid germinal centers. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Dilute in PBS or medium which is identical to that used in the assay system.
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4
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储存条件
- -80 °C
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储存方法
- This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.
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- CENPF (Centromere Protein F (CENPF))
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别名
- CENP-F
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背景
- Centromere protein F,Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. {PubMed:12974617, PubMed:17600710, PubMed:7542657, PubMed:7651420}.,Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.,CENP-F, AH antigen, Kinetochore protein CENPF, Mitosin
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基因ID
- 1063
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NCBI登录号
- NP_057427
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UniProt
- P49454
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途径
- Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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