MGMT 抗体 (AA 8-221)
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- 抗原 See all MGMT 抗体
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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抗原表位
- AA 8-221
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MGMT antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Species Reactivity: Human. Cross-reacts weakly with mouse, rat and hamster. Other species not tested. Molecular Weight: .25 kDa Cellular Location: Nuclear and cytoplasmic.Positive Control: Raji cells, Colon carcinoma.
- 免疫原
- Purified recombinant human MGMT protein.
- 克隆位点
- MT3-1
- 亚型
- IgG1
- Top Product
- Discover our top product MGMT Primary Antibody
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anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 32-210) antibody
MGMT 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1A11F8 unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 101-207) antibodyMGMT 适用: 大鼠 WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibodyMGMT 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (N-Term) antibodyMGMT 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibodyMGMT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 156-182) antibodyMGMT 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibodyMGMT 适用: 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibodyMGMT 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibodyMGMT 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 156-182), (C-Term) antibodyMGMT 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19082 unconjugated
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- 应用备注
- Flow cytometry Immunohistochemistry: (formalin / paraffin) Use at a 1:20 - 1:40 dilution for 60 minutes at room temperature (RT). Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10 - 20 minutes followed by cooling at RT for 20 minutes. Note: IHC requires the use of a high-efficiency detection system. Western blot: Use at 0.5-1 µg/mL for 2 hours at RT. The optimal dilution for a specific application should be determined by the researcher.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 500 µL of monoclonal antibody at 200 µg/mL in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein G chromatography.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- 别名
- MGMT (MGMT 产品)
- 别名
- AGT antibody, AI267024 antibody, Agat antibody, O-6-methylguanine-DNA methyltransferase antibody, MGMT antibody, Mgmt antibody
- 背景
- Cancer chemotherapeutic alkylating agents (e.g. BCNU) act by inducing formation of lethal cross-links at the O6-alkylguanine position in DNA. MGMT (O6-methylguanine-DNA methyltransfer-ase) transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug-resistant.
- 途径
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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