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- 抗原 See all POLR3A 抗体
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
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抗原表位
- Middle Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLR3A antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- POLR3 A antibody was raised against the middle region of POLR3
- 纯化方法
- Affinity purified
- 免疫原
- POLR3 A antibody was raised using the middle region of POLR3 corresponding to a region with amino acids AYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRPLIFDT
- Top Product
- Discover our top product POLR3A Primary Antibody
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anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibody
POLR3A 适用: 人 WB, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (AA 392-632) antibodyPOLR3A 适用: 人 ELISA, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人, 小鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人, 小鼠, 猴 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (C-Term) antibodyPOLR3A 适用: 人, 小鼠, 大鼠, 猴, 犬, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (AA 1-200) antibodyPOLR3A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人, 小鼠, 大鼠, 猴, 犬, 兔, Cow, 小鸡, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: 1 µg/mL
Optimal conditions should be determined by the investigator. - 说明
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POLR3A Blocking Peptide, catalog no. 33R-1627, is also available for use as a blocking control in assays to test for specificity of this POLR3A antibody
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Lyophilized powder. Add distilled water for a 1 mg/mL concentration of POLR0 antibody in PBS
- 浓度
- Lot specific
- 缓冲液
- PBS
- 注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
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- 抗原
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
- 别名
- POLR3A (POLR3A 产品)
- 别名
- 9330175N20Rik antibody, BC053071 antibody, RPC1 antibody, RPC155 antibody, RGD1305574 antibody, ADDH antibody, HLD7 antibody, hRPC155 antibody, polymerase (RNA) III (DNA directed) polypeptide A antibody, RNA polymerase III subunit A antibody, Polr3a antibody, POLR3A antibody
- 背景
- DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
- 分子量
- 156 kDa (MW of target protein)
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