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- 抗原 See all POLR3A 抗体
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLR3A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
- 交叉反应
- 小鼠, 小鸡, 非洲爪蟾, 牛, Xenopus tropicalis
- 交叉反应 （详细）
- Mouse (97 %), Chicken (97 %), Xenopus laevis (94 %), Bovine (98 %), Xenopus tropicalis (94 %)
- 产品特性
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Rabbit Polyclonal antibody to POLR3A (polymerase (RNA) III (DNA directed) polypeptide A, 155 kDa)
POLR3A antibody - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the N-terminus region of human POLR3A. The exact sequence is proprietary.
- 亚型
- IgG
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anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (AA 392-632) antibody
POLR3A 适用: 人 ELISA, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人, 小鼠 ELISA, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人, 小鼠, 猴 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (C-Term) antibodyPOLR3A 适用: 人, 小鼠, 大鼠, 猴, 犬, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (AA 1-200) antibodyPOLR3A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人, 小鼠, 大鼠, 猴, 犬, 兔, Cow, 小鸡, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
- 说明
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Positive Control: HCT116 , SK-N-SH , IMR32 , SK-N-AS
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.49 mg/mL
- 缓冲液
- 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- 抗原
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
- 别名
- POLR3A (POLR3A 产品)
- 别名
- 9330175N20Rik antibody, BC053071 antibody, RPC1 antibody, RPC155 antibody, RGD1305574 antibody, ADDH antibody, HLD7 antibody, hRPC155 antibody, polymerase (RNA) III (DNA directed) polypeptide A antibody, RNA polymerase III subunit A antibody, Polr3a antibody, POLR3A antibody
- 背景
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DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Cellular Localization: Nucleus - 分子量
- 156 kDa
- 基因ID
- 11128
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