SMARCA4 抗体 (N-Term)
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- 抗原 See all SMARCA4 抗体
- SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SMARCA4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- BRG1 Antibody detects endogenous levels of total BRG1.
- 预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human BRG1, corresponding to a region within N-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product SMARCA4 Primary Antibody
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anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (AA 1420-1470), (C-Term) antibody
SMARCA4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p), CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) antibodySMARCA4 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (AA 30-130) antibodySMARCA4 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (C-Term) antibodySMARCA4 适用: 人, 小鼠 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) antibodySMARCA4 适用: 人, 小鼠 WB, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (AA 555-763) antibodySMARCA4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (AA 808-1092) antibodySMARCA4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) antibodySMARCA4 适用: 人 WB, IP 宿主: 小鼠 Monoclonal unconjugated
anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (AA 1352-1386), (C-Term) antibodySMARCA4 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB51961 unconjugated
anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (AA 213-295) antibodySMARCA4 适用: 人, 小鼠, 猴 WB, IF, ICC 宿主: 大鼠 Monoclonal 5B7 unconjugated
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- 应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))
- 别名
- SMARCA4 (SMARCA4 产品)
- 别名
- SMARCA4 antibody, BAF190 antibody, BAF190A antibody, BRG1 antibody, MRD16 antibody, RTPS2 antibody, SNF2 antibody, SNF2L4 antibody, SNF2LB antibody, SWI2 antibody, hSNF2b antibody, brg-1 antibody, Brg1 antibody, HP1-BP72 antibody, SNF2beta antibody, SW1/SNF antibody, b2b692Clo antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody, SMARCA4 antibody, Smarca4 antibody
- 背景
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Description: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (By similarity). Binds to RNA in a promiscuous manner (By similarity). Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities (By similarity).
Gene: SMARCA4
- 分子量
- 185kDa
- 基因ID
- 6597
- UniProt
- P51532
- 途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance
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