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Histone 3 抗体 (H3K27me3)

Cited in 1 publication. This anti-Histone 3 antibody is a 兔 多克隆 antibody detecting Histone 3 in WB, IHC, IF, ELISA, ChIP, IP 和 ICC. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN6266167
发货至: 中国
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Quick Overview for Histone 3 抗体 (H3K27me3) (ABIN6266167)

抗原

See all Histone 3 (H3) 抗体
Histone 3 (H3) (Histone H3 (H3))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • 抗原表位

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    H3K27me3

    特异性

    Tri-Methyl-Histone H3 (Lys27)/H3K27me3 Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys27.

    纯化方法

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    免疫原

    A synthetic methylated peptide derived from human Tri-Methyl-Histone H3 around the methylation site of Lys27.

    亚型

    IgG
  • 应用备注

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • Chen, Liu, Lu, Luo, Wang, Wang, Cai, Yang, Xie, Zhang, Yun: "HBx-mediated decrease of AIM2 contributes to hepatocellular carcinoma metastasis." in: Molecular oncology, Vol. 11, Issue 9, pp. 1225-1240, (2018) (PubMed).

  • 抗原

    Histone 3 (H3) (Histone H3 (H3))

    别名

    HIST3H3

    背景

    Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene: HIST3H3

    分子量

    15kDa

    基因ID

    8290

    UniProt

    Q16695
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