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ATG4D 抗体 (C-Term)

This 兔 多克隆 anti-ATG4D antibody specifically detects ATG4D in WB, ELISA, IHC, IF 和 ICC. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN6259487
发货至: 中国
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中国
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Quick Overview for ATG4D 抗体 (C-Term) (ABIN6259487)

抗原

See all ATG4D 抗体
ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

适用

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人, 小鼠

宿主

  • 65
  • 9
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克隆类型

  • 66
  • 9
多克隆

标记

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This ATG4D antibody is un-conjugated

应用范围

  • 62
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

    • 15
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    C-Term

    特异性

    ATG4D Antibody detects endogenous levels of total ATG4D.

    预测反应

    Pig,Horse,Dog

    纯化方法

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    免疫原

    A synthesized peptide derived from human ATG4D, corresponding to a region within C-terminal amino acids.

    亚型

    IgG
  • 应用备注

    WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • 抗原

    ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

    别名

    ATG4D

    背景

    Description: Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

    Gene: ATG4D

    分子量

    53 kDa

    基因ID

    84971

    UniProt

    Q86TL0

    途径

    Autophagy
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