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- 抗原 See all PRKG1 抗体
- PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))
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抗原表位
- C-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PRKG1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- PRKG1 Antibody detects endogenous levels of total PRKG1.
- 预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human PRKG1, corresponding to a region within C-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product PRKG1 Primary Antibody
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anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) (AA 629-660), (C-Term) antibody
PRKG1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB1046 unconjugated
anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) (AA 73-170) antibodyPRKG1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5E5 unconjugated
anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) (AA 73-170) antibodyPRKG1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2B3 unconjugated
anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) antibodyPRKG1 适用: 人, 小鼠, 大鼠, Pig, Cow, 小鸡, 犬 IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) antibodyPRKG1 适用: 人, 小鼠, 大鼠, Pig WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) (AA 1-312) antibodyPRKG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) (AA 73-171) antibodyPRKG1 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2B3 unconjugated
anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) (AA 657-671) antibodyPRKG1 适用: 人, 小鼠, 大鼠, Pig, Cow, 小鸡, 犬, Bat, 马, 猴, 兔 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) (Center) antibodyPRKG1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, CGMP-Dependent, Type I (PRKG1) antibodyPRKG1 适用: 人 WB, IF, FACS 宿主: 小鼠 Monoclonal 9A4 unconjugated
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- 应用备注
- WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 有效期
- 12 months
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- 抗原
- PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))
- 别名
- PRKG1 (PRKG1 产品)
- 别名
- prkg1 antibody, zgc:63955 antibody, Pkgi antibody, cGk1 antibody, 1 antibody, PKG antibody, PRKG1B antibody, PRKGR1B antibody, cGK antibody, cGK 1 antibody, cGK1 antibody, cGKI antibody, cGKI-BETA antibody, cGKI-alpha antibody, CGKI antibody, cGKI-beta antibody, AW125416 antibody, Gm19690 antibody, Prkg1b antibody, Prkgr1b antibody, Prkg1beta antibody, protein kinase, cGMP-dependent, type Ia antibody, protein kinase, cGMP-dependent, type I antibody, protein kinase, cGMP-dependent, type 1 antibody, prkg1a antibody, PRKG1 antibody, prkg1 antibody, Prkg1 antibody
- 背景
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Description: Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca2+ release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca2+ levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation, decreasing contraction, controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.
Gene: PRKG1
- 分子量
- 76 kDa
- 基因ID
- 5592
- UniProt
- Q13976
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