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CHEK2 抗体 (pThr383)

Name: CHEK2 抗体 (pThr383)
SKU: ABIN6256552
Availability: OutOfStock

This anti-CHEK2 antibody is a 兔多克隆 antibody detecting CHEK2 in WB, ELISA, IHC, IF 和 ICC. Suitable for 人, 小鼠和大鼠.

Western blot analysis of Chk2 phosphorylation expression in UV treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (CHEK2 抗体 (pThr383))

ABIN6267251 at 1/100 staining mouse gastric tissue sections by IHC-P. (CHEK2 抗体 (pThr383))

IHC

ABIN6267251 at 1/100 staining human TB tissue sections by IHC-P. (CHEK2 抗体 (pThr383))

IHC

ABIN6267251 at 1/100 staining rat liver tissue sections by IHC-P. (CHEK2 抗体 (pThr383))

12 images

产品编号 ABIN6256552

发货至: 中国

Datasheet as PDF

Quick Overview for CHEK2 抗体 (pThr383) (ABIN6256552)

抗原

See all CHEK2 抗体

CHEK2 (Checkpoint Kinase 2 (CHEK2))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This CHEK2 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product CHEK2 Primary Antibody

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pThr383

特异性

Phospho-Chk2 (Thr383) Antibody detects endogenous levels of Chk2 only when phosphorylated at Threonine 383.

预测反应

Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human Chk2 around the phosphorylation site of Thr383.

亚型

IgG
anti-Checkpoint Kinase 2 (CHEK2) (pThr387) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (AA 1-543) antibody
CHEK2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (AA 1-543) antibody
CHEK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4B7 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (pSer516) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 犬 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 2H5 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 犬 WB, IHC, IF, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 5C4 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 猴, 犬 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 10G7 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (AA 1-220) antibody
CHEK2 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody
CHEK2 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB19067 unconjugated

应用备注

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:1000, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

CHEK2 (Checkpoint Kinase 2 (CHEK2))

别名

CHEK2

背景

Description: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978).
Gene: CHEK2

分子量

60kDa

基因ID

11200

UniProt

O96017

途径

p53 Pathway, Apoptosis, Cell Division Cycle