我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

MDM2 抗体 (pSer166)

Name: MDM2 抗体 (pSer166)
SKU: ABIN6256167
Availability: OutOfStock

This anti-MDM2 antibody is a 兔多克隆 antibody detecting MDM2 in WB, IHC, ELISA, IF 和 ICC. Suitable for 人, 小鼠和猴.

Western blot analysis of MDM2 phosphorylation expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (MDM2 抗体 (pSer166))

IF/ICC

ABIN6267585 at 1/100 staining human breast carcinoma tissues sections by IHC-P. (MDM2 抗体 (pSer166))

IHC

ABIN6267585 at 1/100 staining Mouse colon tissue by IHC-P. (MDM2 抗体 (pSer166))

IHC

ABIN6267585 staining COS cells by ICC/IF. (MDM2 抗体 (pSer166))

IF/ICC

5 images

产品编号 ABIN6256167

发货至: 中国

Quick Overview for MDM2 抗体 (pSer166) (ABIN6256167)

抗原

See all MDM2 抗体

MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

适用

人, 小鼠, 猴

宿主

兔

克隆类型

多克隆

标记

This MDM2 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product MDM2 Primary Antibody

抗原表位
25

25

13

13

10

10

10

10

9

8

8

5

4

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer166

特异性

Phospho-MDM2 (Ser166) Antibody detects endogenous levels of MDM2 only when phosphorylated at Serine 166

交叉反应

人, 猴, 小鼠

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human MDM2 around the phosphorylation site of Serine 166

亚型

IgG
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 101-200) antibody
MDM2 适用: 人 WB, ELISA, IHC (p), RNAi, PLA 宿主: 小鼠 Monoclonal 1A7 unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 130-230) antibody
MDM2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody
MDM2 适用: 人, 小鼠 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 26-169) antibody
MDM2 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3C1E3 unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 393-424) antibody
MDM2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer186) antibody
MDM2 适用: 人 WB, ELISA, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) antibody
MDM2 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 119-438) antibody
MDM2 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 200-430) antibody
MDM2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 26-169) antibody
MDM2 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4A8D12 unconjugated

应用备注

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C.Stable for 12 months from date of receipt

有效期

12 months
抗原

MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

别名

MDM2

背景

Description: E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity).
Gene: MDM2

分子量

55+90kDa

基因ID

4193

UniProt

Q00987

途径

p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway