电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

MDM2 抗体 (Center)

MDM2 适用: 人, 小鼠 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN2854782
发货至: 中国
  • 抗原 See all MDM2 抗体
    MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
    抗原表位
    • 26
    • 25
    • 14
    • 11
    • 10
    • 10
    • 10
    • 9
    • 9
    • 8
    • 8
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center
    适用
    • 194
    • 79
    • 55
    • 11
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠
    宿主
    • 169
    • 28
    • 1
    克隆类型
    • 169
    • 29
    多克隆
    标记
    • 99
    • 13
    • 9
    • 9
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This MDM2 antibody is un-conjugated
    应用范围
    • 125
    • 77
    • 64
    • 37
    • 28
    • 27
    • 27
    • 18
    • 18
    • 13
    • 10
    • 9
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)
    交叉反应
    猫, 犬, 猪
    交叉反应 (详细)
    Cat (95 %), Dog (91 %), Pig (93 %)
    产品特性
    Rabbit Polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)
    MDM2 antibody
    纯化方法
    Purified by antigen-affinity chromatography.
    免疫原
    Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.
    亚型
    IgG
  • 应用备注
    Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:1000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:1000*
    说明

    Positive Control: H1299 , HCT116 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , mouse MDM2-transfected 293T

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.74 mg/mL
    缓冲液
    1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原
    MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
    别名
    MDM2 (MDM2 产品)
    别名
    ACTFS antibody, HDMX antibody, hdm2 antibody, zgc:109834 antibody, 1700007J15Rik antibody, AA415488 antibody, Mdm-2 antibody, MDM2 proto-oncogene antibody, MDM2 proto-oncogene S homeolog antibody, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antibody, MDM2 oncogene, E3 ubiquitin protein ligase antibody, transformed mouse 3T3 cell double minute 2 antibody, MDM2 antibody, mdm2.S antibody, Mdm2 antibody, mdm2 antibody
    背景
    This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

    Cellular Localization: Nucleus , nucleoplasm , Cytoplasm , nucleolus
    分子量
    55 kDa
    基因ID
    4193
    途径
    p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway
You are here:
客服