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Calponin 抗体

适用: 人, 大鼠 IHC (p), IF 宿主: 小鼠 Monoclonal CLPN5-1 unconjugated
产品编号 ABIN6252460
发货至: 中国
  • 抗原 See all Calponin products
    Calponin
    适用
    • 39
    • 33
    • 24
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    • 5
    • 3
    • 1
    • 1
    • 1
    人, 大鼠
    宿主
    • 24
    • 16
    小鼠
    克隆类型
    • 28
    • 12
    单克隆
    标记
    • 25
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
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    This Calponin antibody is un-conjugated
    应用范围
    • 18
    • 18
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    • 12
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    • 9
    • 2
    • 1
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    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant human protein was used as the immunogen for the Calponin antibody.
    克隆位点
    CLPN5-1
    亚型
    IgG1 kappa
  • 应用备注
    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Optimal dilution of the Calponin antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    Calponin
    Abstract
    Calponin 产品
    别名
    CalPoNin antibody, cpn-1 antibody, cpn-2 antibody
    背景
    Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
    基因ID
    1264
    UniProt
    P51911
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