Calponin 抗体
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- 抗原 See all Calponin products
- Calponin
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适用
- 人, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Calponin antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant full-length human protein (CNN1/832) and crude human uterus extract (CALP) were used as the immunogen for the Calponin antibody cocktail.
- 克隆位点
- CNN1-832-CALP
- 亚型
- IgG
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anti-Calponin (AA 1-298) antibody
适用: 人 IHC (p), WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2F5-1H4 unconjugated
anti-Calponin (N-Term) antibody适用: 人, 大鼠, 小鼠, 犬, 猴, Cow, 马, 绵羊 WB 宿主: 兔 Polyclonal unconjugated
anti-Calponin antibody适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 兔 Monoclonal CNN1-1408R unconjugated Recombinant Antibody
anti-Calponin antibody适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 兔 Monoclonal CNN1-1408R unconjugated Recombinant Antibody
anti-Calponin antibody适用: 人 IHC (p), IF, FACS 宿主: 兔 Monoclonal RMCN1-1 unconjugated Recombinant Antibody
anti-Calponin antibody适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 小鼠 Monoclonal CNN1-832 unconjugated
anti-Calponin antibody适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 小鼠 Monoclonal CNN1-832-CALP unconjugated
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- 应用备注
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Optimal dilution of the Calponin antibody cocktail should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Calponin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Calponin
- Abstract
- Calponin 产品
- 别名
- CalPoNin antibody, cpn-1 antibody, cpn-2 antibody
- 背景
- Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
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