PLAP 抗体
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- 抗原 See all PLAP (ALPP) 抗体
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PLAP antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant full-length human ALPP protein was used as the immunogen for the placental Alkaline Phosphatase antibody.
- 克隆位点
- ALPP-870
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product ALPP Primary Antibody
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- 应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the placental Alkaline Phosphatase antibody should be determined by the researcher.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- 抗原
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
- 别名
- Placental Alkaline Phosphatase (ALPP 产品)
- 别名
- ECK0378 antibody, JW0374 antibody, psiA antibody, ALP antibody, PALP antibody, PLAP antibody, PLAP-1 antibody, ILC antibody, CTACK antibody, ESkine antibody, Akp2 antibody, alp antibody, zgc:56672 antibody, DDBDRAFT_0205491 antibody, DDBDRAFT_0231570 antibody, DDB_0205491 antibody, DDB_0231570 antibody, ALPI antibody, Actn2lp antibody, Alp antibody, bacterial alkaline phosphatase antibody, alkaline phosphatase, placental antibody, alkaline phosphatase, placental type antibody, C-C motif chemokine ligand 27 antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase antibody, alkaline phosphatase, intestinal antibody, PDZ and LIM domain 3 antibody, phoA antibody, ALPP antibody, LOC100436725 antibody, CCL27 antibody, alpl antibody, alp antibody, ALPI antibody, Pdlim3 antibody, Alpp antibody
- 背景
- Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
- 基因ID
- 250
- UniProt
- P05187
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